Because our neuroscience faculty members are affiliated with a number of different departments, a variety of research facilities are available. Students interested in the molecular basis of neural function may take advantage of up-to-date equipment for cloning and sequencing DNA, purifying large samples of protein, and analyzing primary, secondary, and tertiary protein structures. Students whose primary interests lie at the cellular and systems level have access to a wide range of neurophysiological instrumentation, including patch clamp and single-unit recording techniques, computer modeling facilities, neuronal imaging set-ups, anatomical tracing reagents and computer-controlled stimulation systems. Those interested in cognitive and behavioral neuroscience work at labs with electrical, optical, and fMRI technology readily available. Doctoral candidates interested in clinical issues will find a full range of patient populations and modern medical instrumentation at our on-campus teaching hospitals and veterinary medicine clinics.
Cell and Immunobiology Core
The Cell and Immunobiology Core Facility provides custom production of monoclonal antibodies, flow cytometric services, and tissue culture reagents. The Core is located in the Medical Sciences Building and is hosted by the Department of Molecular Microbiology and Immunology.
The Cell Culture core facility at the MU Dalton Cardiovascular Research Center provides consultation on methods for dispersing cells from in-tact tissues for short-term use as suspensions or single cells, and for long-term maintenance of cells in culture. Investigators have direct access to the cell culture laboratory, and the core staff provides instruction in specific cell culture methods.
Charles W. Gehrke Proteomics Center
The Proteomics Center provides synthesizes custom peptides, sequences proteins, and provides HPLC and capillary electrophoretic analyses. The core is located in the Christopher S. Bond Life Sciences Center.
The DNA Core provides services in DNA sequencing, DNA fragment analysis, genotyping, genomic variation analysis, and gene expression. The DNA Core also maintains the campus Enzyme Freezer Program, which contains an extensive inventory of restriction enzymes, PCR and qPCR reagents, DNA and RNA purification kits, and various chemicals and biochemicals. Robotic instrumentation also is available for high throughput plasmid isolation, colony picking, and genomic library management. The core is located in the Christopher S. Bond Life Sciences Center and hosted by the Department of Molecular Microbiology and Immunology.
The imaging core at the Dalton Cardiovascular Research Center is equipped with an Olympus inverted microscope with fluorescence capabilities, a Photometrics digital camera, Dage video camera, dissecting scope and light boxes. Histology, immunofluorescence, autoradiography, dynamic imaging of cellular processes and documentation of gels are all performed in this facility. Investigators have direct access to the imaging core facility, and instruction in the use of equipment and software is available.
Molecular Cytology Core
The Molecular Cytology Core is a resource for all types of light microscopy, immunocytochemistry, in situ hybridization techniques, and general scientific image analysis and processing. Instrumentation available for client use includes two confocal microscopes, micro-injection system, epi-fluorescence stereoscope, conventional wide-field microscopes, paraffin microtome, cryostat, and ultramicrotome. The core is located and hosted by the Christopher S. Bond Life Sciences Center.
Nuclear Magnetic Resonance Core
The Nuclear Magnetic Resonance Core provides equipment maintenance and infrastructure support of research projects that require NMR or for investigators who want to use NMR for structural elucidation and study of chemical and biological reactions. Instrumentation at the Nuclear Magnetic Resonance Core facility includes an 800 MHz NMR spectrometer. The core is located in the Chemistry Building and is hosted by the Molecular Biology Program.
Electron Microscopy Core
The Electron Microscopy Core supports research that requires scanning or transmission electron microscopy. Services include scanning and transmission electron microscopy, immunoelectron microscopy, x-ray microanalysis, digital imaging and analysis, and preparation of materials and biological specimens. The core is located in the Veterinary Medicine Building and is hosted by the Department of Veterinary Pathobiology.
Structural Biology Core
The Structural Biology Core provides instrumentation and faculty assistance with crystallography, dynamic light scattering, NMR, and peptide synthesis. Researchers who understand the important link between macromolecular structure and function can explore how to get structural information on proteins of interest and which method is best for their needs. The core is located in the Chemistry Building and is hosted by the Molecular Biology Program.
The Proteomics Center provides advanced technologies in protein separation and mass spectrometry identification for researchers at the University of Missouri-Columbia. The Center accepts any non-hazardous, non-pathogenic, and non-radioactive samples for analyses. These samples can be whole organism (cell pellets, seedlings, etc.), tissue (biopsies, roots, leaves, etc.), or purified protein (cell lysate, precipitated protein, IPs, gel bands/spots).
Transgenic Animal Core
The transgenic Animal Core provides many services, including but not limited to chromosome counting, generation of transgenic mice on FVB and c57BI6 strains using client’s DNA, and genotyping assays. The laboratory facilities include dedicated laboratories for cell culture and micromanipulation. The core is located in the Veterinary Medicine Building and is hosted by the Division of Animal Sciences.
Center for Translational Neuroscience
The MU Center for Translational Neuroscience serves as a home base for researchers from such programs as biochemistry, pathology and anatomical sciences, biological sciences, psychological sciences, neurology and neurosurgery. Center experts serve as consultants and support teams for other scientists involved in related research.
University of Missouri Bioinformatics Consortium
The University of Missouri Bioinformatics Consortium provides an integrated array of high performance computing and communications products and related services to users, including supercomputing-class hardware, software, mass storage systems, file storage areas, consulting services, and training.
Informatics Research Core
The Informatics Research Core Facility serves the bioinformatics needs of researchers at MU. It provides custom web-enabled software development, analysis of high-throughput data, and assembly and annotation of genome-wide sequence data. The core is located in the Bond Life Sciences Center.